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Dilutions
Introduction
Introduction to Dilutions
Standard, serial, ratio and experimental dilutions
Dilutions
Introduction
Standard v. Serial Dilutions
Making up the right volume for an experiment
How do I perform dilution calculations?
Worked Examples - what volume of the stock solution is needed?
Example 1: What volume of a 2 M stock solution would you need to make a 1 l solution with a final concentration of 1 M?
Example 2: What volume of a 1 M stock solution would you need to make a 1 l solution with a final concentration of 0.1 M?
Example 3: What volume of a 1 M stock solution would you need to make a 0.1 l solution with a final concentration of 0.1 M?
Example 4: What volume of a 1 M stock solution would you need to make a 100 ml solution with a final concentration of 100 mM?
Example 5: What volume of a 1 mM stock solution would you need to make a 10 ml solution with a final concentration of 10 µM?
Example 6: What volume of a 100 mM stock solution would you need to make a 250 ml solution with a final concentration of 0.1 µM?
Worked Examples - what is the final concentration of a solution?
Example 1: What would be the final concentration of a solution if you took 1 l of a 1 M stock and you made it up to a final volume of 2 l?
Example 2: What would be the final concentration of a solution if you took 0.5 l of a 2 M stock and you made it up to a final volume of 1 l?
Example 3: What would be the final concentration of a solution if you took 200 ml of a 0.5 M stock and you made it up to a final volume of 0.5 l?
Example 4: What would be the final concentration of a solution if you took 200 ml of a 0.5 M stock and you made it up to a final volume of 0.5 l?
Example 5: What would be the final concentration of a solution if you took 200 µl of a 0.5 µM stock and you made it up to a final volume of 10 ml?
Test your skills your dilution skills
Test your skills
Serial Dilutions
Introduction
The importance of getting serial dilutions correct
Standard v. Serial Dilutions
Making up the right volume
Calculating serial dilutions
Serial Dilutions - worked examples
Example 1: What would be the concentration in the 1st dilution of a 10 fold dilution if the starting concentration was 1 M?
Example 2: What would be the concentration in the 2nd dilution of a 10 fold dilution if the starting concentration of the stock solution was 1 M?
Example 3: What would be the concentration in the 10th dilution of a 10 fold dilution if the starting concentration of the stock solution was 1 M?
Example 4: What would be the concentration in the 8th dilution of a 5 fold dilution if the starting concentration of the stock solution was 100 mM?
Test your understanding of serial dilutions
Test your understanding of serial dilutions
Ratio dilutions (1 in 1000 type)
Introduction to ratio dilutions
Ratio Dilutions - worked examples
Example 1: You need to produce 10 ml of a solution and the stock needs to be diluted 1:1000. How much of the stock do you need?
Example 2: You need to produce 5 ml of a solution and the stock needs to be diluted 1:5000. How much of the stock do you need?
Example 3: You need to produce 10 ml of a solution and the stock needs to be diluted 3:1000. How much of the stock do you need?
Example 4: You need to produce 18 ml of a solution and the stock needs to be diluted 7:6000. How much of the stock do you need?
Test your understanding of ratio dilutions
Test your understanding of ratio dilutions
Example 5: What volume of a 1 mM stock solution would you need to make a 10 ml solution with a final concentration of 10 µM?
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